Chiefland is a city in Levy County, Florida, United States. The population was 2,245 at the 2010 census. Chiefland calls itself the "Gem of the Suwannee Valley" and was incorporated in 1929.
Chiefland is located at 29°28′54″N 82°51′44″W (29.481801, -82.862097). According to the United States Census Bureau, the city has a total area of 16.1 square miles (42 km²), all land. Chiefland is at the junction of U.S. Highways Alternate 27, 19, and 98. US 129 was converted into a separate junction in the early 21st century. It is 30 miles (48 km) southwest of Gainesville.
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